What is the benefit of using fluorescent tags in sequencing methods?

Using fluorescent tags in sequencing methods is beneficial primarily because they allow for the visualization of nucleotides during the analysis phase. Fluorescent dye molecules can be attached to specific nucleotides, enabling their detection through fluorescence microscopy or other imaging techniques. This is particularly useful in sequencing because it allows for real-time monitoring of the sequencing process, as each nucleotide incorporated into the growing DNA strand emits a specific color of light corresponding to that nucleotide.

This capability of visualizing nucleotides as they are added aids in accurately determining the sequence of the DNA or RNA being analyzed. With this approach, it becomes easier to identify the specific order of bases, enhancing the resolution and precision of sequencing results. The ability to visualize the nucleotides in real time contributes significantly to the efficiency and accuracy of sequencing technologies, such as Sanger sequencing and next-generation sequencing.

Other choices may highlight important aspects of genetics or sequencing methods, but they do not directly address the key role of fluorescent tags in enabling the visualization of nucleotides, which is central to their function in sequencing applications.